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Sino Biological gp2 mouse mab
Gp2 Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gp2+mouse+mab/Ebola+virus+EBOV+(subtype+Zaire%2C+strain+H%2Esapiens-wt%2FGIN%2F2014%2FKissidougou-C15)+GP2+%2F+Glycoprotein+Antibody%2C+Mouse+MAb/pmc12148371-23-1-5
Average 93 stars, based on 1 article reviews

gp2 mouse mab - by Bioz Stars, 2026-09

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Article Title: Soluble CD4 inhibits Ebola virus infection by targeting endosomal receptor-binding site
Article Snippet: EBOV, GP2 mouse Mab , Sino Biological Inc. , 40442-MM28.

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(A) Cartoon describing the experimental set-up. Aged mice (∼20 months old) were housed for 6 weeks in cages of used bedding that had previously housed young mice. Bedding was replaced twice weekly. Control aged mice were housed in clean cages. (B) Whole-mount immunostaining of GP2+ M cells (green) in Peyer’s patches from young, aged and aged mice given young bedding. Counterstain, F-actin (blue). Scale bar, 100 µm. Broken line, the boundary of the FAE. (C) Quantitation of the number of GP2+ M cells in Peyer’s patches from mice from each group. Each point represents an individual FAE. Horizontal line, median. n=12-16/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (D) IHC detection of Spi-B+ cells (green) in the FAE of Peyer’s patches from mice in each group. Nuclei detected using DAPI (blue). Scale bar, 20 µm. Broken line, the apical surface of the FAE. (E) Quantitation of the number of Spi-B+ cells in the FAE of Peyer’s patches from mice from each group. Each point represents an individual section. Horizontal line, median. n=17-31/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (F) IHC detection of CCL20 (red) in the FAE of Peyer’s patches from mice from each group. Nuclei detected using DAPI (blue). Scale bar, 20 µm. Broken line, the apical surface of the FAE. (G) Quantitation of the % area CCL20+ immunostaining in the FAE of Peyer’s patches from mice from each group. Each point represents an individual FAE section. Horizontal line, median. n=11-17/group from 3-4 mice. Statistical differences determined by one-way ANOVA.

Journal: bioRxiv

Article Title: Microbial Stimulation Reverses the Age-Related Decline in M Cells in Aged Mice

doi: 10.1101/2020.02.17.943514

Figure Lengend Snippet: (A) Cartoon describing the experimental set-up. Aged mice (∼20 months old) were housed for 6 weeks in cages of used bedding that had previously housed young mice. Bedding was replaced twice weekly. Control aged mice were housed in clean cages. (B) Whole-mount immunostaining of GP2+ M cells (green) in Peyer’s patches from young, aged and aged mice given young bedding. Counterstain, F-actin (blue). Scale bar, 100 µm. Broken line, the boundary of the FAE. (C) Quantitation of the number of GP2+ M cells in Peyer’s patches from mice from each group. Each point represents an individual FAE. Horizontal line, median. n=12-16/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (D) IHC detection of Spi-B+ cells (green) in the FAE of Peyer’s patches from mice in each group. Nuclei detected using DAPI (blue). Scale bar, 20 µm. Broken line, the apical surface of the FAE. (E) Quantitation of the number of Spi-B+ cells in the FAE of Peyer’s patches from mice from each group. Each point represents an individual section. Horizontal line, median. n=17-31/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (F) IHC detection of CCL20 (red) in the FAE of Peyer’s patches from mice from each group. Nuclei detected using DAPI (blue). Scale bar, 20 µm. Broken line, the apical surface of the FAE. (G) Quantitation of the % area CCL20+ immunostaining in the FAE of Peyer’s patches from mice from each group. Each point represents an individual FAE section. Horizontal line, median. n=11-17/group from 3-4 mice. Statistical differences determined by one-way ANOVA.

Article Snippet: To detect M cells by whole-mount immunostaining Peyer’s patches were first fixed using BD Cytofix/Cytoperm (BD Biosciences), and then immunostained with rat anti-mouse GP2 mAb (MBL International, Woburn, MA).

Techniques: Immunostaining, Quantitation Assay

(A) Histological detection of fluorescent 200 nm nanobeads (arrows) in the SED region of young, aged and aged mice given young bedding. Scale bar, 100 µm. Broken line, the apical surface of the FAE. (B) Quantitation of the number of 200 nm fluorescent nanobeads in the SED of Peyer’s patches from each group. Each point represents an individual section. Horizontal line, median. n=51-94/group from 3-4 mice. Statistical differences determined by Kruskal-Wallis. (C) Corrrelation between GP2+ M cell-density in the FAE and the number of fluorescent 200 nm nanobeads in the SED. Statistical significance determined by Spearman correlation. (D) IHC detection on CD11c+ MNP (green) and CD68+ MNP (red) in the FAE and SED of Peyer’s patches from mice from each group. Nuclei detected using DAPI (blue). Scale bar, 50 µm. Broken line, basal surface of the FAE. (E) The % area of CD11c+ immunostaining in the SED of mice from each group. Each point represents an individual section. Horizontal line, median. n=16-30/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (F) Quantitation of the number of CD11c+ MNP in the FAE of mice from each group. Each point represents an individual section. Horizontal line, median. n=18-33/group from 3-4 mice. Statistical differences determined by Kruskal-Wallis. (G) Comparison of the % area of CD68+ immunostaining in the SED of mice from each group. Each point represents an individual section. Horizontal line, median. n=16-30/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (H) Specific anti-horse spleen ferritin (HSF) IgA levels were determined by ELISA 2 weeks after oral HSF administration in faecal homogenates from aged and aged mice given young bedding. Data presented as mean ± SEM. n=3-4/group. Statistical differences determined by two-way ANOVA.

Journal: bioRxiv

Article Title: Microbial Stimulation Reverses the Age-Related Decline in M Cells in Aged Mice

doi: 10.1101/2020.02.17.943514

Figure Lengend Snippet: (A) Histological detection of fluorescent 200 nm nanobeads (arrows) in the SED region of young, aged and aged mice given young bedding. Scale bar, 100 µm. Broken line, the apical surface of the FAE. (B) Quantitation of the number of 200 nm fluorescent nanobeads in the SED of Peyer’s patches from each group. Each point represents an individual section. Horizontal line, median. n=51-94/group from 3-4 mice. Statistical differences determined by Kruskal-Wallis. (C) Corrrelation between GP2+ M cell-density in the FAE and the number of fluorescent 200 nm nanobeads in the SED. Statistical significance determined by Spearman correlation. (D) IHC detection on CD11c+ MNP (green) and CD68+ MNP (red) in the FAE and SED of Peyer’s patches from mice from each group. Nuclei detected using DAPI (blue). Scale bar, 50 µm. Broken line, basal surface of the FAE. (E) The % area of CD11c+ immunostaining in the SED of mice from each group. Each point represents an individual section. Horizontal line, median. n=16-30/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (F) Quantitation of the number of CD11c+ MNP in the FAE of mice from each group. Each point represents an individual section. Horizontal line, median. n=18-33/group from 3-4 mice. Statistical differences determined by Kruskal-Wallis. (G) Comparison of the % area of CD68+ immunostaining in the SED of mice from each group. Each point represents an individual section. Horizontal line, median. n=16-30/group from 3-4 mice. Statistical differences determined by one-way ANOVA. (H) Specific anti-horse spleen ferritin (HSF) IgA levels were determined by ELISA 2 weeks after oral HSF administration in faecal homogenates from aged and aged mice given young bedding. Data presented as mean ± SEM. n=3-4/group. Statistical differences determined by two-way ANOVA.

Techniques: Quantitation Assay, Immunostaining, Enzyme-linked Immunosorbent Assay

(A) NMDS plot comparing faecal microbiota structure between mice from each age group. n=7-14/group. Faecal microbiotas were significantly different between young mice and each group of aged mice (P=0.002; HOMOVA). (B) Bacterial diversity in faeces from young, aged and aged mice given young bedding was compared using the Shannon index. Horizontal line, median. n=7-14/group. Statistical differences determined by one-way ANOVA (Kruskal-Wallis). (C) Bubble plot showing the relative abundance of distinct bacterial families in the faecal microbiotas of mice from each group. n=7-14/group. Statistical differences for each family determined by one-way ANOVA or Kruskal-Wallis where appropriate. (D-F)The relative abundance of (D) Bifidobacteriaceae, (E) Turicibacteriaceae , and (F) Verruocomicrobiaceae in faeces of young, aged and aged mice given young bedding. Data presented as mean ± SEM. n=7-14/group. Statistical differences determined by Kruskal-Wallis. (G-I) Correlation between the relative abundance of Verruocomicrobiaceae in faeces and (G) the density of FAE GP2+ M cells, (H) the number of fluorescent 200 nm nanobeads in the SED, and (I) the level of antigen-specific faecal IgA. Statistical significance determined by Spearman correlation. (J-L) Correlation between the relative abundance of Turicibacteriaceae in the faeces and (J) the density of FAE GP2+ M cells, (K) the number of fluorescent 200 nm nanobeads detected in the SED, and (L) the level of antigen-specific faecal IgA. Statistical significance determined by Spearman correlation.

Journal: bioRxiv

Article Title: Microbial Stimulation Reverses the Age-Related Decline in M Cells in Aged Mice

doi: 10.1101/2020.02.17.943514

Figure Lengend Snippet: (A) NMDS plot comparing faecal microbiota structure between mice from each age group. n=7-14/group. Faecal microbiotas were significantly different between young mice and each group of aged mice (P=0.002; HOMOVA). (B) Bacterial diversity in faeces from young, aged and aged mice given young bedding was compared using the Shannon index. Horizontal line, median. n=7-14/group. Statistical differences determined by one-way ANOVA (Kruskal-Wallis). (C) Bubble plot showing the relative abundance of distinct bacterial families in the faecal microbiotas of mice from each group. n=7-14/group. Statistical differences for each family determined by one-way ANOVA or Kruskal-Wallis where appropriate. (D-F)The relative abundance of (D) Bifidobacteriaceae, (E) Turicibacteriaceae , and (F) Verruocomicrobiaceae in faeces of young, aged and aged mice given young bedding. Data presented as mean ± SEM. n=7-14/group. Statistical differences determined by Kruskal-Wallis. (G-I) Correlation between the relative abundance of Verruocomicrobiaceae in faeces and (G) the density of FAE GP2+ M cells, (H) the number of fluorescent 200 nm nanobeads in the SED, and (I) the level of antigen-specific faecal IgA. Statistical significance determined by Spearman correlation. (J-L) Correlation between the relative abundance of Turicibacteriaceae in the faeces and (J) the density of FAE GP2+ M cells, (K) the number of fluorescent 200 nm nanobeads detected in the SED, and (L) the level of antigen-specific faecal IgA. Statistical significance determined by Spearman correlation.

Techniques:

(A) Whole-mount immunostaining of GP2+ M cells (green) in Peyer’s patches from PBS- and flagellin-treated aged mice. Counterstain, F-actin (blue). Scale bar, 100 µm. Broken line, the boundary of the FAE. (B) Quantitation of the number of GP2+ Mcells in Peyer’s patches from mice from each group. Each point represents an individual FAE. Horizontal line, median. n=17-23/group from 3-4 mice. Statistical difference determined by t-test. (C) IHC detection on Spi-B+ cells (green) in the FAE of Peyer’s patches from mice from each group. Nuclei detected with DAPI (blue). Scale bar, 20 µm. Broken line, the apical surface of the FAE. (D) Quantitation of Spi-B+ cells in the FAE of Peyer’s patches from mice in each group. Each point represents an individual section. Horizontal line, median. n=14-24/group from 3-4 mice. Statistical difference determined by Mann-Whitney. (E) Quantitation of the number of 200 nm fluorescent nanobeads in the SED of PBS- and flagellin-treated aged mice 24 h after orally exposure. Data presented as mean ± SEM. n=53-90/group from 3-4 mice. Statistical difference determined by Mann-Whitney. (F) IHC detection on CD11c+ (green) and CD68+ MNP (red) in the FAE and SED in mice from each group. Nuclei detected with DAPI (blue). Scale bar, 50 µm. Broken line, the basal surface of the FAE. Arrows, CD11c+ cells in FAE. (G-H) The % area of CD11c+ (G) and CD68+ (H) immunostaining in the SED of mice in each group. Each point is from an individual section. Horizontal line, median. n=10-23/group from 3-4 mice. Statistical difference determined Mann-Whitney. (I) Number of CD11c+ cells in the FAE of Peyer’s patches from PBS- and flagellin-treated aged mice. Each point is from an individual section. Horizontal line, median. n=8-23/group from 3-4 mice. Statistical difference determined by t-test. (J) Quantitation of the number of 200 nm fluorescent nanobeads in the SED of PBS or flagellin-treated M cell-deficient RANK ΔIEC mice 24 h after oral exposure. M cell-sufficient RANK F/F mice were used as a positive control. Each point is from an individual section. Horizontal line, median. n=31-41/group from 3 mice. Statistical differences determined by Kruskal-Wallis.

Journal: bioRxiv

Article Title: Microbial Stimulation Reverses the Age-Related Decline in M Cells in Aged Mice

doi: 10.1101/2020.02.17.943514

Figure Lengend Snippet: (A) Whole-mount immunostaining of GP2+ M cells (green) in Peyer’s patches from PBS- and flagellin-treated aged mice. Counterstain, F-actin (blue). Scale bar, 100 µm. Broken line, the boundary of the FAE. (B) Quantitation of the number of GP2+ Mcells in Peyer’s patches from mice from each group. Each point represents an individual FAE. Horizontal line, median. n=17-23/group from 3-4 mice. Statistical difference determined by t-test. (C) IHC detection on Spi-B+ cells (green) in the FAE of Peyer’s patches from mice from each group. Nuclei detected with DAPI (blue). Scale bar, 20 µm. Broken line, the apical surface of the FAE. (D) Quantitation of Spi-B+ cells in the FAE of Peyer’s patches from mice in each group. Each point represents an individual section. Horizontal line, median. n=14-24/group from 3-4 mice. Statistical difference determined by Mann-Whitney. (E) Quantitation of the number of 200 nm fluorescent nanobeads in the SED of PBS- and flagellin-treated aged mice 24 h after orally exposure. Data presented as mean ± SEM. n=53-90/group from 3-4 mice. Statistical difference determined by Mann-Whitney. (F) IHC detection on CD11c+ (green) and CD68+ MNP (red) in the FAE and SED in mice from each group. Nuclei detected with DAPI (blue). Scale bar, 50 µm. Broken line, the basal surface of the FAE. Arrows, CD11c+ cells in FAE. (G-H) The % area of CD11c+ (G) and CD68+ (H) immunostaining in the SED of mice in each group. Each point is from an individual section. Horizontal line, median. n=10-23/group from 3-4 mice. Statistical difference determined Mann-Whitney. (I) Number of CD11c+ cells in the FAE of Peyer’s patches from PBS- and flagellin-treated aged mice. Each point is from an individual section. Horizontal line, median. n=8-23/group from 3-4 mice. Statistical difference determined by t-test. (J) Quantitation of the number of 200 nm fluorescent nanobeads in the SED of PBS or flagellin-treated M cell-deficient RANK ΔIEC mice 24 h after oral exposure. M cell-sufficient RANK F/F mice were used as a positive control. Each point is from an individual section. Horizontal line, median. n=31-41/group from 3 mice. Statistical differences determined by Kruskal-Wallis.

Techniques: Immunostaining, Quantitation Assay, MANN-WHITNEY, Positive Control

(A) IHC detection of CCL20 (red) in the FAE of Peyer’s patches from PBS- and flagellin-treated aged mice. Nuclei detected with DAPI (blue). Scale bar, 20µm. Broken line shows the apical FAE surface. (B) Quantitation of the % area CCL20+ immunostaining in the FAE of Peyer’s patches from mice in each group of panel A. Each point is from an individual FAE. Horizontal line, median. n=10-22/group from 3-4 mice. Statistical difference determined by Mann-Whitney. (C-F) In vitro enteroids prepared from small intestinal crypts were treated with RANKL, flagellin or both. Expression of Ccl20 (C), Spib (D), Sox8 (E) and Gp2 (F) was compared by qRT-PCR 24 or 72 h later. Mean expression levels were normalized so that untreated enteroids at 24 h equalled 1.0. Data expressed as mean ± SEM. n=3. Statistical differences determined by two-way ANOVA. (G-H) Comparison of Tlr5 and Gp2 mRNA expression (G) in individual cell populations in deep CAGE sequence data from the FANTOM5 project of the FANTOM consortium , and (H) in published mRNA-seq data from isolated GP2+ M cells (GSE108529; ).

Journal: bioRxiv

Article Title: Microbial Stimulation Reverses the Age-Related Decline in M Cells in Aged Mice

doi: 10.1101/2020.02.17.943514

Figure Lengend Snippet: (A) IHC detection of CCL20 (red) in the FAE of Peyer’s patches from PBS- and flagellin-treated aged mice. Nuclei detected with DAPI (blue). Scale bar, 20µm. Broken line shows the apical FAE surface. (B) Quantitation of the % area CCL20+ immunostaining in the FAE of Peyer’s patches from mice in each group of panel A. Each point is from an individual FAE. Horizontal line, median. n=10-22/group from 3-4 mice. Statistical difference determined by Mann-Whitney. (C-F) In vitro enteroids prepared from small intestinal crypts were treated with RANKL, flagellin or both. Expression of Ccl20 (C), Spib (D), Sox8 (E) and Gp2 (F) was compared by qRT-PCR 24 or 72 h later. Mean expression levels were normalized so that untreated enteroids at 24 h equalled 1.0. Data expressed as mean ± SEM. n=3. Statistical differences determined by two-way ANOVA. (G-H) Comparison of Tlr5 and Gp2 mRNA expression (G) in individual cell populations in deep CAGE sequence data from the FANTOM5 project of the FANTOM consortium , and (H) in published mRNA-seq data from isolated GP2+ M cells (GSE108529; ).

Techniques: Quantitation Assay, Immunostaining, MANN-WHITNEY, In Vitro, Expressing, Quantitative RT-PCR, Sequencing, Isolation

Comparison of M cell and follicular dendritic cell (FDC) status in the secondary lymphoid tissues of CXCR5 ΔDC mice and CXCR5 F/F control mice. (A) Peyer's patches were whole-mount immunostained to detect M cells (GP2 + cells, green). F-actin (blue) was used as a counterstain. The broken line indicates the boundary of the follicle associated epithelium (FAE). V, villi. (B) The number of GP2 + M cells/FAE was similar in Peyer's patches from each mouse group ( P = 0.710). (C) Morphometric analysis suggested the size of the FAE area was also similar in each mouse group ( P = 0.265 [Student t test]; data were derived from 2 to 20 FAE/mouse [ n = 6 to 8 mice/group]). IHC comparisons of CD35 (magenta) and PrP C (green) expression by FDC in the Peyer's patches (D), mesenteric lymph nodes (MLN) (G), and spleens (J) of CXCR5 F/F and CXCR5 ΔDC mice are shown. The sizes of the FDC in the Peyer's patches (E), MLN (H), and (K) spleens of mice from each group were estimated by morphometric analysis of the area of CD35 + immunostaining. The abundance of PrP C expressed by the FDC in Peyer's patches (F), MLN (I), or spleens (L) from each mouse group was estimated by morphometric analysis of the number of PrP C+ pixels within each FDC network. Data were derived from 2 to 10 FDC/mouse ( n = 6 to 8 mice/group).

Journal: Journal of Virology

Article Title: Oral Prion Disease Pathogenesis Is Impeded in the Specific Absence of CXCR5-Expressing Dendritic Cells

doi: 10.1128/JVI.00124-17

Figure Lengend Snippet: Comparison of M cell and follicular dendritic cell (FDC) status in the secondary lymphoid tissues of CXCR5 ΔDC mice and CXCR5 F/F control mice. (A) Peyer's patches were whole-mount immunostained to detect M cells (GP2 + cells, green). F-actin (blue) was used as a counterstain. The broken line indicates the boundary of the follicle associated epithelium (FAE). V, villi. (B) The number of GP2 + M cells/FAE was similar in Peyer's patches from each mouse group ( P = 0.710). (C) Morphometric analysis suggested the size of the FAE area was also similar in each mouse group ( P = 0.265 [Student t test]; data were derived from 2 to 20 FAE/mouse [ n = 6 to 8 mice/group]). IHC comparisons of CD35 (magenta) and PrP C (green) expression by FDC in the Peyer's patches (D), mesenteric lymph nodes (MLN) (G), and spleens (J) of CXCR5 F/F and CXCR5 ΔDC mice are shown. The sizes of the FDC in the Peyer's patches (E), MLN (H), and (K) spleens of mice from each group were estimated by morphometric analysis of the area of CD35 + immunostaining. The abundance of PrP C expressed by the FDC in Peyer's patches (F), MLN (I), or spleens (L) from each mouse group was estimated by morphometric analysis of the number of PrP C+ pixels within each FDC network. Data were derived from 2 to 10 FDC/mouse ( n = 6 to 8 mice/group).

Article Snippet: Briefly, Peyer's patches were fixed with BD Cytofix/Cytoperm (BD Biosciences, Oxford, UK), and subsequently immunostained with rat anti-mouse GP2 monoclonal antibody (MAb; MBL International, Woburn, MA).

Techniques: Comparison, Control, Derivative Assay, Expressing, Immunostaining

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